Luciferase activity in vivo imaging
This mini review provides an overview of the luciferases currently being applied in in vivo imaging applications along with the toolbox of approaches that continue to expand the capabilities of in vivo BLI. Advances in the in vivo BLI field have created luciferase proteins with expanded wavelength emission profiles, stronger and more stable signal generation, substrate-independent real-time expression, and proximity-based expression characteristics that are providing innovative tools for preclinical diagnostics, drug discovery, and toxicology research. Multimodality molecular imaging of stem cells therapy for stroke. Dual luciferase labelling for non-invasive bioluminescence imaging of mesenchymal stromal cell chondrogenic differentiation in demineralized bone matrix scaffolds. Similar to beetle luciferases, bacterial luciferase generates light in an ATP-dependent manner in the presence of long-chain aldehyde, FMNH 2and molecular oxygen Tissue engineering of vascularized cardiac muscle from human embryonic stem cells. Mol Microbiol.
Firefly Luciferase pyralis, is the most studied and well characterized bioluminescent reporter gene . The toolbox of in vivo BLI includes an inventory of luciferase compounds .
Noninvasive optical imaging of firefly luciferase reporter gene. Monitoring of Renilla Luciferase Activities in-vitro and in-vivo promising bioluminescence reporter gene that can be used for noninvasive optical imaging.
A diamino-phenylpropyl-aminoluciferin DAL substrate was ultimately developed as a BioLeT probe for the targeting of biological nitric oxide.
Luciferase Reporters Thermo Fisher Scientific US
Transcriptional and functional profiling of human embryonic stem cell-derived cardiomyocytes. Fluorescence by Unbound Excitation from Luminescence Fluorescence by unbound excitation from luminescence FUEL is similar to BRET in that it uses the emission spectra of a bioluminescent donor to activate the excitation spectra of a fluorescent acceptor.
Biochem Biophs Res Commun 1 — Identification, characterization, and regulation of a cluster of genes involved in carbapenem biosynthesis in Photorhabdus luminescens. J Biolumin Chemilumin. One in vivo study investigated the methods by improving cardiomyoblast survival and biodistribution, including overexpression of BCL2 genes [ 71 ].
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Multiple luciferases can also be used for multiplexed examination of complex interactions involving multiple protein partners simultaneously in the same subject 12 A dual-color luciferase assay system reveals circadian resetting of cultured fibroblasts by co-cultured adrenal glands. In vivo visualization of embryonic stem cell survival, proliferation, and migration after cardiac delivery.
Video: Luciferase activity in vivo imaging In Vitro Bioluminescence Imaging on the IVIS Platform
Paulmurugan R, Gambhir SS. Aminoluciferins extend firefly luciferase bioluminescence into the near-infrared and can be preferred substrates over d-luciferin. Lee DS.
In Vivo Cell Tracking with Bioluminescence Imaging
The application of transcription factor activated luciferase reporter cassettes in vitro is widespread but potential for in vivo application has not. Reporter gene expression was monitored with an in vivo imaging system. In Vivo Bioluminescent Imaging of MDR1-Luc Reporter Mice.
Comparison of human optimized bacterial luciferase, firefly luciferase, and green fluorescent protein for continuous imaging of cell culture and animal models.
Bacterial bioluminescence: its control and ecological significance. This allows supply of the activation signal to be decoupled from measurement of the emission signal to potentially reduce background and increase sensitivity.
The Expanding Toolbox of In Vivo Bioluminescent Imaging
Kim SB, Izumi H. Overexpression, purification, and analytical application of a bioluminescent reporter for DNA hybridization.
Video: Luciferase activity in vivo imaging PerkinElmer's IVIS Spectrum Pre-clinical In Vivo Imaging System
BRET3: a red-shifted bioluminescence resonance energy transfer BRET -based integrated platform for imaging protein-protein interactions from single live cells and living animals.